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簡要描述：免 疫 原:KLH conjugated synthetic peptide derived from human RAG2: 451-527/527
產(chǎn)品型號： GOY-01K0001廠商性質(zhì)：科研更新時間：2023-10-24 00:00:00訪 問 量：292產(chǎn)品分類
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重組激活基因2蛋白抗體
| 產(chǎn)品名稱 | 英文名稱 | 貨號 |
| 重組激活基因2蛋白抗體 | RAG2 | GOY-01K0001 |
商品屬性：
英文名稱:RAG2
中文名:重組激活基因2蛋白抗體
別 名:RAG 2; RAG-2; RAG2; RAG2_HUMAN; Recombination activating gene 2; V(D)J recombination activating protein 2; V(D)J recombination-activating protein 2.
研究領(lǐng)域細(xì)胞生物: 免疫學(xué) 表觀遺傳學(xué)
抗體來源:Rabbit
克隆類型:Polyclonal
交叉反應(yīng):(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
產(chǎn)品應(yīng)用:ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量:58kDa
細(xì)胞定位細(xì)胞核
性 狀:Liquid
濃 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived from human RAG2: 451-527/527
亞 型:IgG
純化方法:affinity purified by Protein A
緩 沖 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產(chǎn)品介紹:
| Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1. |
多克隆抗體制備：
我公司合成抗原，保證陽性血清和抗體ELISA效價達(dá)到1：32000，由蛋白抗原制備的多抗，保證WB檢測結(jié)果是陽性。技術(shù)服務(wù)實行分步報價，按節(jié)點收費，為客戶節(jié)約成本。
鼠單克隆抗體制備:
客戶可根據(jù)項目實際需求情況靈活選擇進(jìn)行，我公司將為您的項目提供專業(yè)的建議。在制備過程中，與客戶互動驗證，確保最終制備的抗體ELISA陽性血清檢測達(dá)到合同要求的同時，也能滿足客戶抗體最終應(yīng)用驗證需求。技術(shù)服務(wù)實行分步報價，按節(jié)點收費，為客戶節(jié)約成本。
重組抗體表達(dá)服務(wù):
重組抗體技術(shù)使制備人源化抗體和人源抗體成為可能，而這是其他常規(guī)的多克隆或單克隆抗體制備方法無法做到的。我公司已搭建好的抗體測序、重組抗體表達(dá)等先進(jìn)平臺，能為客戶提供各種類型的重組抗體表達(dá)服務(wù)，包含鼠源單克隆抗體構(gòu)建及表達(dá)、更換恒定區(qū)亞型的嵌合抗體表達(dá)，抗體人源化改造及表達(dá)等服務(wù)。
單項免疫學(xué)及檢測服務(wù):
我公司也為客戶提供單項免疫學(xué)和檢測服務(wù)，如動物免疫、動物飼養(yǎng)、抗體修飾服務(wù)（如生物素，F(xiàn)ITC，HRP等的標(biāo)記）、以及ELISA，WB，IHC，DB等檢測服務(wù)。
抗體定制服務(wù):
已注冊商標(biāo)，主要用于抗體藥物發(fā)現(xiàn)和開發(fā)，是我公司針對有特定應(yīng)用要求和各種模型測試的高端客戶量身定制的專業(yè)服務(wù)，主要面向工業(yè)客戶。
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